HBsAg / HBsAb Tests

HBV ( Hepatitis B Virus ) , that cause of chronic hepatitis is classified as a hepadnavirus. It is a major factor in the eventual development of liver disease and hepato cellular carcinoma in the individuals. HBV has three major antigens consists of HBsAg , HBcAg , and HBeAg.
HBsAg is firstly appear when patient is infected by hepatitis B virus and usually detectable 2 - 6 weeks in advance of clinical and biochemical evidence of hepatitis and persist throughtout the clinical course of the disease but typically disappears by the sixth month after exposure. Antibody to HBsAg is first detected at a variable period after the disappearance of HBsAg. This rapid diagnostic of HBV infection is essential to patient management and disease control.
The development of rapid immuno-chromatographic assay of HBsAg is an initial screening test for the detection of HBV infection from patient's serum / plasma specimens.
The development of a rapid immuno-chromatographic assay of HBs Antibody is an initial screening test for the detection of abtibody to HBs as low as 10 mIU / ml from patient's serum / plasma.

Specification :

1. HBsAg

• Sample : Serum /plasma
  
• Volume : 3 drops
• Reading time : 10 - 30 minutes
• Cut off value : 1 ng/ml
• Sensitivity > 99%
• Specificity > 96.7%
• Long Expiry Date
• Package 25 tests or 50 tests

1. HBsAb

• Sample : Serum/Plasma
• Volume : 3 drops
• Reading time : 10 - 30 minutes
• Cut off value : 10 mIU/mL
• Sensitivity > 99%
• Specificity > 98.7%
• Long Expire Date
• Package 25 tests or 50 tests

Procedure :

1. Add 3 drops of sample into sample well.
2. Read the result within 30 minutes after adding sample.
   

Malaria Combo Antigen Test

Malaria is one of the world's most prevalent parasitic diseases and ranks third in the world among major infectious diseases in terms of mortality. The protozoal parasites that cause malaria are from the plasmodium genus. Four species of plasmodium protozoa cause malaria : Plasmodium Falciparum, Plasmodium Vivax, Plasmodium Malariae, and Plasmodium Ovale.
Plasmodium Falciparum accounts for the majority of infections and is the most lethal.
This differentiating , combination malaria test is a rapid , in-vitro immunodiagnostic test for the detection of circulating malaria antigens in whole blood. The test uses antibodies that are specific for the histidine-rich protein 2 antigen ( HRP-2 ) of malaria plasmodium falciparum and plasmodium aldolase for the detection of all malaria plasmodium species.

Specification :

• Sample : Whole Blood
• Volume : 5 uL whole blood
• Reading time : 5 - 30 minutes
• Sensitivity : 100% P.Falciparum , 97% P.Other
• Specivicity : > 98% P.Falciparum , 98% P.Other
• Long expiry date
• Package 25 tests or 50 tests

Procedure :

1. Add to sample pad 5 uL whole blood then one drop of the lysing / wash reagent to the sample pad on top of the whole blood.
2. Add 5 drops of the lysing / wash reagent to the buffer well.
3. Using a timer , allow the reaction to proceed for 15 minute.
4. Read the result within 30 minutes.
   

HCG ( Pregnancy Test )

Human Chorionic Gonadotropin ( HCG ) is a glycoprotein hormone produced by the developing placenta shortly after fertilization. In normal pregnancy , HCG can be detected in both urine and serum as 7 to 10 days after conception during early gestational growth, make it an excellent marker for the early detection of pregnancy. The HCG rapid test is a chromatographic immunoassay for the qualitative detection of human chorionic gonadotropin (HCG) in urine or serum to aid in early of detection of pregnancy.

Specification :

• Sample : Urine
• Volume : 3 drops
• Reading time : 5 - 30 minutes
  
• Sensitivity : 25 mIU / ml
• Package 25 tests or 50 tests

Procedure :

1. Add 3 drops of sample into sample well.
2. Read the result within 30 minutes after adding sample.
   

Drug Abuse Test

The Drug Abuse Rapid Test is a competitive chromatographic immunoassay based one step in vitro test. It is designed for qualitative determination of their metabolites in human urine specimens above a certain cut-off level within 5~30 minutes.

Specification :

• Sample : Urine
• Volume : 3 drops
• Reading time : 5-30 minutes
• Long expiry date
• Package 25 Tests or 50 tests.

Products :

• Methamphetamine Cut off value : 500 ng/ml
• Cocaine Cut off value: 300 ng/ml
• Marijuana (THC) Cut off value: 50 ng/ml
• Morphine Cut off value: 300 ng/ml
• Amphetamine Cut off value: 1000 ng/ml
• Benzodiazephines Cut off value: 300 ng/ml
• MDMA Cut off value: 500 ng/ml

Procedure :

• Add 3 drops of samples into sample well.
• Read the result within 30 minutes after adding sample.
  

RAPID TEST

Rapid Tes adalah :

• Test one-step assay yang sangat mudah penggunaannya.
• Tes tidak invasif, untuk mendeteksi beberapa penyakit dan kondisi tertentu.
• Menggunakan prinsip membrane-based immunoassays/imunochromatography yang menggunakan monoclonal / polyclonal antibody reaksi.
• Hasil dapat dibaca secara visual.

Goal Rapid Tes :

• Umumnya digunakan untuk screening tes.
• Menghemat waktu serta biaya dibandingkan dengan tes diagnostik lainnya seperti Elisa.
• Dapat membantu para dokter mendiagnosa berbagai macam penyakit dengan cepat sehingga penanganan pengobatan dapat dilakukan secepatnya.
• Tidak membutuhkan alat khusus serta tenaga ahli khusus , dapat dilakukan dimanapun juga.
• Harga lebih ekonomis.

Komponen Rapid Tes :

• Membrane : nitrocellulose.
• Sample Pad.
• Conjugate Pad : dilapisi dengan detektor reagent ( Conjugate Ag/Ab-indicator complex), spesifik terhadap analyte. Sebagai indicator dapat digunakan Latex ,Enzym ,Carbon ,Colloidal sol partikel.
• Tes Band : dilapisi dengan capture reagent pada membrane , spesifik terhadap analyte.
• Control Band : dilapisi dengan Anti-detector Ab pada membrane , berfungsi sebagai kontrol validnya tes.
• Soak Pad : lapisan penyerap untuk cairan sample yang berlebih.

Prinsip Rapid Tes :

1. Indirect
2. Sandwich
3. Competition

DAFTAR PRODUK RAPID TEST

Drug Abuse :

1. Methamphetamine
2. Cocaine
3. THC
4. Morphine
5. Amphetamine
6. Barbiturate
7. Benzodiazepine
8. PCP
9. Amp , Morp , THC ( 3 in 1 )
10. Amp,Morp,THC,Meth,Benz (5 in 1 )

Blood Transmission :

1. HIV I & II Antibody
2. HCV Antibody
3. HBsAg Antigen
4. HBsAb Antibody
5. Syphilis Antibody

Infectious Disease :

1. Tuberculosis Antibody
2. H. Pylori Antibody
3. Dengue IgM / IgG
4. Malaria Antigen

Others :

1. Troponin I
2. HCG
3. PSA
4. FOB (Fecal Occult Blood)
5. FOBT (Fecal Occult Blood + Transferin )
6. Urine Analysis-10
7. Urine Analysis- 3
8. Urine Analysis- 1
9. Widal , dll